> DreamSkin® trials and data

DreamSkin® Health garments have been tested with groups of volunteers, and are currently undergoing further clinical trials with patients suffering from eczema and dermatitis.

  • > The effectiveness of DreamSkin® Health garments at protecting the skin from external irritant damage

     

     

    Click to view data

    Summary

    DreamSkin® treated fabrics offer protection from external irritants. After 24 hours of artificial skin inflammation, skin covered with a DreamSkin® health fabric showed over 40% greater cells alive when compared to untreated fabric.

     

    Methodology

    The skin protection test of DreamSkin® textiles has been carried out utilising JIS L 1918 – Japanese Industrial Standard approved test for primary irritation tests.

    Artificial skin pieces were covered with either DreamSkin® treated test fabrics or untreated fabrics.

    Sodium Dodecyl Sulphate – a known irritant - was then dropped onto the test fabric and left to cure at 37 degrees centigrade for 24 hours.

    Following removal of the test fabric, the artificial skin was then stained and analysed for live vs dead cells.

    SDS containing Artificial sweat

    Ring

    Test Piece

    Skin Surface

    Real Skin

    Culture base

    V = (A1 - b) / (A2 - b) x 100

     

    V: cell alive %

    A1: sample light wave absorbency

    A2: control light wave absorbency

    b: extracted solution light wave absorbency

    This test was repeated on five samples for each treatment. (20 total)

    Cell Alive count was set as 100% for skin pieces which are not treated but follow the dyeing and extraction process. This sets a relative 100% as a baseline comparison and removes the analysis variable.

     

    Results

    The percentage of living cells is replicated below.

    Statistical Analysis

    Endpoint: the percentage of cells alive at 24 hours after being dropped with SDS.

     

    (i) Summary

    (ii) Analysis

    (iii) Pairwise comparisons of Treatments from ANCOVA model

    Summary

    The results of an analysis of covariance (ANCOVA) model shows that treatment has a significant impact on the fit of the model.

     

    The individual pairwise comparisons demonstrate that both types of DreamSkin®  yield significantly higher percentage of cells alive than the untreated fabric.

     

    Improvement for DreamSkin® 2 compared to an untreated fabric is in the order of 40%

    (95% CI: 31 - 49) more cells alive after 24 hours.

     

     

  • > The ability of DreamSkin® Health garments to increase moisture recovery in damaged skin

     

     

    Click to view data

    Summary

    The data shows a relative moisture recovery rate for DreamSkin® as 3 times that of untreated fabric and 1.6 times that of squalane treated fabric.

     

    Test Methodology

    Recovery of Trans-Epidermal water loss (TEWL) measures the rate that skin recovers water lost through irritation or skin complaints.

    The methodology is presented clearly below, and was carried out on a test group of 15 volunteers – 5 in each test group.

     

     

     

    Recovery of Transepidarmal Water Loss (TEWL) and

    Water Content in Keratinized Layer (WCKL)

    1.   Measurement of TEWL and WCKL on normal Skin. (inside of forearm)

    2.   Artificial inflammation using anionic surfactant (closed patch for 12hr)

    3.   Measurement of TEWL and WCKL on rough Skin. (inside of forearm)

    6.   Steps 4 and 5 were repeated for 4 days continuously.

     

          Then the TEWL index and the WCKL index were calculated.

    4.   Wearing of tights treated with DreamSkin®

          (6hr/day)

    5.   Measurement of TEWL and WCKL after wearing tights

    TEWL

     

    (TEWL after wearing - TEWL on normal skin)

    (TEWL on rough skin - TEWL on normal skin)

    x 100

    1-

    {

    }

    WCKL

     

    (WCKL after wearing - WCKL on normal skin)

    (WCKL on rough skin - WCKL on normal skin)

    x 100

    1-

    {

    }

    Results

    Statistical Analysis

    Analysis of Recovery rate of Trans-Epidermal Water Loss (TEWL)

    Endpoint: the Area Under the Curve (AUC) for Recovery Rate over the 4 days following application of SDS on the skin.

     

    (i) Summary

    (ii) Analysis

    (iii) Pairwise comparisons of Treatments from ANCOVA model

    Summary

    The curve shows the pattern in each group, and includes a numerical summary of the AUC from which analysis is calculated.

     

    The results of an analysis of covariance (ANCOVA) model shows that the DreamSkin® treatment has a significant impact on the rate TEWL recovery rate.

     

    The three individual comparisons show that each pair of treatments is significantly different, with DreamSkin® giving a significantly higher cumulative recovery over the 4 days in comparison to either of the other groups.

     

    In this sample, the data shows a relative recovery rate for DreamSkin® as 3 times that of untreated fabric and 1.6 times that of squalane treated fabric.

     

     

  • > The effectiveness of DreamSkin® Health garments at enhancing the recovery rate of damaged skin

     

     

    Click to view data

    Summary

    The test data shows a recovery rate for Dreamskin Health garments of approximately 3.3 times that of squalane treated fabric.

     

    Test Methodology

    Water Content in Keratinized Layer (WCKL) is a widely used measure of the state of health of skin – in cosmetics as well as dermatology.

     

    The methodology is presented clearly below, and was carried out on a test group of 15 volunteers – 5 in each test group.

     

    Fabrics treated with squalane were used as a comparison as it is a widely used emollient and cosmetic component.

     

     

     

    Recovery of Transepidarmal Water Loss (TEWL) and

    Water Content in Keratinized Layer (WCKL)

    1.   Measurement of TEWL and WCKL on normal Skin. (inside of forearm)

    2.   Artificial inflammation using anionic surfactant (closed patch for 12hr)

    3.   Measurement of TEWL and WCKL on rough Skin. (inside of forearm)

    6.   Steps 4 and 5 were repeated for 4 days continuously.

     

          Then the TEWL index and the WCKL index were calculated.

    4.   Wearing of tights treated with DreamSkin®

          (6hr/day)

    5.   Measurement of TEWL and WCKL after wearing tights

    TEWL

     

    (TEWL after wearing - TEWL on normal skin)

    (TEWL on rough skin - TEWL on normal skin)

    x 100

    1-

    {

    }

    WCKL

     

    (WCKL after wearing - WCKL on normal skin)

    (WCKL on rough skin - WCKL on normal skin)

    x 100

    1-

    {

    }

    Results

    Analysis of Water Content in Keratinized Layer (WCKL)

    Endpoint: the Area Under the Curve (AUC) for Recovery Rate over the 4 days following application of SDS on the skin.

     

    (i) Summary

    (ii) Analysis

    (iii) Pairwise comparisons of Treatments from ANCOVA model

    Summary

    The curve shows the pattern of WCKL recovery in each group, and includes a numerical summary of the AUC upon which the analysis is performed.

     

    The comparison of the two groups shows a significant difference between the treatments, with DreamSkin® giving a significantly higher cumulative recovery over the 4 days in comparison to the squalane treated fabric.

     

    The data suggests a recovery rate for DreamSkin® of approximately 3.3 times that of the squalane treated fabric.